Movement of Cortical Actin Patches in Yeast
Waddle et al., 1996. J. Cell Biol. 132: 861-870.
Online Journal
Abstract
In yeast, actin forms patches associated with the plasma membrane. Patch distribution correlates with polarized growth during the cell cycle and in response to external stimuli. Using green fluorescent protein fused to capping protein to image actin patches in living cells, we find that patches move rapidly and over long distances. Even patches in clusters, such as at the incipient bud site, show movement. Patches move independently of one another and generally over small distances in a local area, but they can also move larger distances, including through the mother-bud neck. Changes in patch polarization occur quickly through the cell cycle. These observations provide important new parameters for a molecular analysis of the regulation and function of actin.
Movies (on YouTube)
Some of the Figures (2, 3, 5 and 6) are available as movies, which provide far more information than the single images. Additional movies, not included as part of the manuscript, are also available.
Figures As Movies
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Figure 2B | Figure 3, Row 1 | Figure 3, Row 2 |
| Figure 3, Row 3 | Figure 3, Row 4 | Figure 3, Row 5 | Figure 3, Row 6 |
| Figure 3, Row 7 | Figure 5A | Figure 5B | Figure 6 |
Movies of Patch Movement
| One Cell, Medium Bud |
One Cell, Small Bud |
Two Cells, Medium Buds |
| Cytokinesis Ring and Buds | Cytokinesis Ring | |
Additional Figure: Patches Do Not Move in Permeabilized Cells
- Patches in Permeabilized Cells.
Legend. Patches do not move in permeabilized cells. Panel A shows sequential frames from a rapid acquisition movie at a single focal plane of a medium-budded cell, collected at 0.4 sec intervals. The actin patch distribution does not change from frame to frame (compare with Fig.3) . Panel B shows actin patch disribution at 15 sec intervals in a medium-budded cell. Each image is a 2-D projection of images collected at all Z-axis planes. Note that patch distribution does not change even for the whole minute (compare to Fig.2A).
